Composite

Part:BBa_K2173002:Design

Designed by: Chandel Angad Singh   Group: iGEM16_IIT_Delhi   (2016-10-21)


LuxI_LuxR_mrfp1(plux)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 879
    Illegal NheI site found at 902
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2514
    Illegal AgeI site found at 2626
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1860


Design Notes

We used LuxI ,LuxR and mRFP1 with Deg tags and after each part there was a double terminator.


Source

Vibrio fischeri,

References